齊口裂腹魚和巖原鯉野生種群遺傳多樣性研究.pdf

1、I’引。牛，齊口裂腹魚和巖原鯉野生種群遺傳多樣性研究StudiesongeneticdiversityofwildpopulationsofPrenant’sschizothoracin(Schizothoraxprenanti)androckcarp(Procyprisrabaudi)博士后姓名：宋昭彬流動站(一級學科)名稱：四川大學生物學專業(yè)(二級學科)名稱：動物學研究工作起始時間2002年12月1口研究工作期滿時間2005年6月2

2、0日四川大學2005年6月AbstractPrenant’sschizothoracins(Schizothoraxprenanti)werecollectedfromYalongRiverandMinRiverandrockcarps(ProcyprisrabaudilwerecollectedfromHejiangsectionoftheYangtzeRiverin2003—2004Thegeneticdiversityandpop

3、ulationstructureofthesetwospecieswasanalysedusingAFLPandmitochondrialDNAcontrolregionTheprimaryresultsareasfollowsAtotalof621lociwereamplifiedusing7pairsofAFLPprimercombinationsin45individualsofPrenant’sschizothoracin，of

4、which8728％werepolymorphicThegeneticdistancesbetweenpopulationsfromQingyiRiver(DuoyingandBaoxing)andfromYalongRiver(Mull)werethefarthest，whilethedistancebetweenthetwopopulationsfromQingyiRiverwasthenearestTheorderfromthep

5、opulationwiththehighestintrapopulationdistancetotheonewiththelowestdistancewasMuli，EbianShimianflungRiver)，DuoyingandBaoxingTheaverageheterozygosityofpopulationDuoying，Muli，EbianShimian，andBaoxingwasO237900330，O262100421

6、，0285600483，andO2385O0512，respectivelyClusteranalysiswithUPGMAmethodindicatedthatthegeneticrelationshipbetweenpopulationfromQingyiRiverandTungRiverwasmuchnearedthanthatbetweenpopulationfromQingyiRiverandYalongRiverTheest

7、imatedFstvalueaveragedoverallpolymorphiclociwas01276，indicatingamoderatedegreeofgeneticdifferentiationamongthepopulationsThecompletelengthofmtDNAcontrolregioninPrenant’sschizothoracinwas932—937bp，inwhichthelengthsofETASd

8、omain，centraldomainandCSBdomainwere25lbp，326hpand362bp，respectivelyTheTAS，CSBD，CSB—E，CSB—F，CSBl，CSB2andCSB3wereidentifiedincontrolregionSequenceanalysisofthe908bpofmtDNAcontrolregiondisclosed78variablesitesin41prenant’ss

9、chizothoracinindividuals，whichdefinedatotalof29haplotypesandtwodivergentcladesThevariablesitesprimarilydistributedinETASandcentraldomainThegeneticdistancesbetweenpopulationBaoxingandMuliwerethefarthest，whilethedistancebe

10、tweenthepopulationDuoyingandEbianShimianwasthenearestTheestimatedFstvaluewas01656，indicatingamoderatedegreeofgeneticdifferentiationamongthepopulationsAtotalof841lociwereamplifiedin16rockcarpindividualsusing10pairsofprime