苦皮藤內(nèi)生真菌As菌株農(nóng)藥活性成分初步研究.pdf

1、分類號(hào)：S182139一UDC；盟盟—一密級(jí)：公玨學(xué)校代碼：10712研究生學(xué)號(hào)：SY20040752西北農(nóng)林針捉大學(xué)2007屆攻讀碩士學(xué)位研究生學(xué)位(畢業(yè))論文苦皮藤內(nèi)生真菌As菌株農(nóng)藥活性成分初步研究學(xué)科專業(yè)研究方向研究生指導(dǎo)教師完成時(shí)間一蕉L葒堂——一叢墊壹堡jL強(qiáng)一一立蔓彗熬握至QQZ生魚旦一一中國陜西楊凌STUDIESONPESTICIDALINGREDIENTSOFSTRAINASOFTHEENDOPHYTICFUN(、II

2、NCE“S豫己靨彳ⅣG阮彳丁【靨ABSTRACTInthecourseofscreeningIlCWpestieidaleompotmds，StrainAs，鼬EndophytiefungushasbeenisolatedfromCelastrusangulatusIntillspaperthetaxonomyofstrainAstogetherwiththefungicdalactivityofit’smetabolitespurif

3、icationofactivecompoundsandop“ttmizationoffermentationconditionswillbedescribednlemainresultsa∞8Sfollows：1ThefimgieidalactivitiesofthemctabolitesofthestrainAshavebeentestedT1leresultsshowedthattheactiveingredientsinthemy

4、eeliumcouldbeex幢actedbyethylaeemteormethanol，butmethanolwasthenlolleappropriatesolventForValsamaltandBotryt括Citleteamyeeliumgrowthinhibitionw勰observedattheeoneenttationof4000蚺g／mLtheinhibitoryratiowasabove80lmecnt；ForCur

5、vulavialunataFusariumcoPr1deunlAlternarialongipessporegerminationinhibitionwasobservedattheconcentrationof5000峙‘mL“。，theinhibitoryratiowasalsoabove80percentmresultsofpottestindieatexlthatthemethanolexllactsofmyeeliumcoul

6、deffectivelycontrolofwheatpowderymildewtheprotectiveandtherapeuticetficaeyWClteallabove65％attheexmeentrafionof4000嵋‘mL一2Bymorphologicaltaxonomymeans，theaerialmyecliummaeroeonidiamieroeonidiaandcolonymorphologycharacteris

7、ticsofstrainAswasobservedByeomparisedwithrelatedclassifysystemstrainAsWaSidentifiedasFusariumoxysporum3Bymeansofopensilicagel，maeroporousresineolunmehromatograplayandpreparative唧LC，threecompounds，ZOl，Z02andZ03，Weleisolat

8、edfromthemethanolexlxaetsoffermentationproductsofstrainAsn圮sturetl艙characteristicofcompoundZ02WelrecarriedbyI／VMS1HNMlland”CNM喂teelmologybutthesttllCt珊eelucidationw孫m伍cultandunfinishedbyDOW111est／tletureofcompoundZ0landZ

9、03couldnotbetestedfor∞appropriatesolventAttheconcenllstionof200lag‘mL一Z01hadnOevidentfungicidalactivityZ02andZ03hadanevidentfungicidalactivityagainstFusariumcoeruleumCurvulavialunataExserohilumturcicumandBotrytiscinercab

10、yIlleall8ofsporegerminationForZ02thevalueofECsoanainstCurvulavialunataWaS86631tg’mL一411磚optimumeompositionofculturemediumandfermentationconditionsoftheswainAsWeredemonstratedbythetestofunifactorandortlaogonaldesignrespec

11、tivelyn圯optimumculturemediumeomistcdofglucose2％，potato20％，pcptoneO5％，KzIIP04O1％，MgS0401％andoriginaJpH607011圯seedingageWaS9611Inoeulumvolumewas5enlriesperlOOmLmediumA250raLflaskcontaininglOOmLmediumwasetdtivatedat26℃for16