大鴇東方亞種遺傳多樣性的分析.pdf

1、東北林業(yè)大學(xué)碩士學(xué)位論文大鴇東方亞種遺傳多樣性的分析姓名：劉鑄申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：特種經(jīng)濟(jì)動(dòng)物飼養(yǎng)指導(dǎo)教師：田秀華白素英20060601AbsⅡaclAbstractGrcatbustard(0協(xié)細(xì)婦)isliStedaSaf：irSt—ratingpmtected陸dillChiⅡaaIldCⅡESII11lenumbefofgrcatbustard(D出加砌劬DM七田isdccreasingrecennyinCh協(xié)aInorde

2、rtof砌thereasonofpopulationdedinea芏ldassistconservation，tIlegeneticdiversityofgreatbus協(xié)rdwasanalyzedbymicm阻telliteDlNAandmtDNAc0Ⅱtmlrc西onmarkcrshstudyusingmicrosatelliteDNAmarkers，meemi口0satellitesfrom緲atbust盯d(D￡缸砌)柚d13m

3、icrosatemtes舶mH0ubmbustard(劬腸呻勘廊肼咖腸耙)weredetecledin47舒eatbustards(D￡匆6D哪趕fEi曲tmiaDsatelliteslociwercpol阿oIphic，3lociofwhichwerclowpolyInorphic姐dtlle姍lailling5lociwerehi曲lypoIyIno吶icIhehetcrozygosity慨)ofthe8lodranged如mO04

4、35to10000，averagingO6595perloctls，theP01ymopllismInfo衄atioⅡcontaⅡt口Ic)ofthe8loci砌gedfmm00416toO8520，avera百ng05497perlocus，aⅡdtllee疵c咖enumberofalleles但)of8lodrangedf如m104t0746，361allelesperlocus0naverageTheobservedgcnotyp

5、e丘equenciesat4lociwere∞nSistentwithHardy—weinbe唱Equilibrium(H、ⅣE)andtlleremaining4lociweresi印ificantlydcviatedfIomHwEIIlstlldyof0t方6DM北越usingmtDNAcontmlre酉onmarkers，tllreercgionsandtlletot“re舀onofmtDNAcontrolrcgionI趾d11w

6、ereanalyzedAmpli血ationofCtrIa眥trIIoHhad8h印lotypes，NudeotidediVersity㈣wasO00328，Haplotypedivefsity(d)was0622，AVeragenumbefofdi船rcnces∞w船1116；AInp監(jiān)cationofL438但772hadn’tmutation；AmpuficationofLCR2棚cR8onlyhad1mutation；111et

7、otalre西onofctrIaL小cR8had7haplotypes，NudeotidediVcrsity㈣wasO00174，Haplotypediversity(d)wasO718，AVeragenumberofdifferences(I()was1359neresultsshowthatgeneticdiversityofD丘咖6D橢iislowefthanDt缸rc如thIou曲compareofmanyaspectsThis

8、maybecausedbythefewertotalnumber，geneticbotⅡeⅡeckinhistoryaIldconccntratedgeo舯phicaldis仃ibutjonnonhwest0fSongliaoplain卸dWulaIlgchabuplateaubreedingareasareaeVolutioⅡarysi印ificaIltunittW0brcedjngareashaveownhaplotype，then

9、adIIlinjsterthemas晰oconservationuni￡sIIlviewofmefactthatthent咖的M強(qiáng)七村areinimminentdanger，first，musttakeimportancetopmtectionofwinteringareas；Next，musttTyourbesttopmtecttheimportambreeding甜eas；Finally，establishesapopulation

10、iIldudingexistiIlggeneticdiVersityforfeintmduction，thusen曲leittogetrid掣aduallyisinimminentdallger，acllieVesthebuStardspeciesprotectionthegoalKeywordsgreatbustard，D出船rda由6D礎(chǔ)越microsatellites，mtDNAcomrolregion，geneticdivers