高速逆流色譜分離制備無(wú)花果葉和長(zhǎng)松蘿中主要活性成分的研究.pdf

1、中國(guó)農(nóng)業(yè)科學(xué)院碩士學(xué)位論文高速逆流色譜分離制備無(wú)花果葉和長(zhǎng)松蘿中主要活性成分的研究姓名：遲春艷申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：農(nóng)產(chǎn)品加工與貯藏工程指導(dǎo)教師：石波20080601AbstractInthispaperlightpetroleum(bp：60℃90℃)wasusedtoextractFicuscaricaLleavesandUsnea10ngissima，andthenevaporatedtogetthecrudeextractHi

2、gh—speedcountercurrentChromatography(HSCCC)wasappliedfortheisolationandpurificationofpsoralenandbergaptenfromtheplantFicuscaricaLleavesforisolationandpurificationofusnicacidfromUsnea10ngissimaThetwophasesolventsystemfors

3、eparatingpsoralenandbergaptenfromtheplantFicuscaricaLleavesbyHSCCCwasnHexaneethylacetatemethanol—water(1：1：1：1，v／v)。ThetwophasesolventsystemforseparatingusnicacidfromUsnea10ngissimabyHSCCCwasnHexaneacetonitrile—ethylacet

4、ate—water(8：7：5：08v／v)20ml／minflowrateand800rpmrevolutionspeedwerethebestoptimumconditionsforFicuscaricaLleaves’purification15ml／minflowrateand600rpmrevolutionspeedwerethebestoptimumconditionsforUsnea10ngissima’Spurifica

5、tion44mgofpsoralenand2。1mgofbergaptenwereobtainedfrom200mgofF／cuscaricaLleavescrudeextractinonestepseparation112mgofusnicacidwasobtainedfrom25mgofUsnea10ngissimacrudeextractThepuritiesofpsoralenbergaptenandusnicaciddeter

6、minedbyHPLCwhichindicatethatpsoralenat991％bergaptenat982％purityandusnicacidabove99％Thestructuresofpsoralenbergaptenandusnicacidwereidentifiedby1HNMR，13C—NMRandMSThecrudeextractsofFicuscaricaLleavesby80％ethanolandUsnea10n

7、gissimabylightpetroleum(bP：60℃一90。C)wereusedtofeedlittlemice，themice’SgrowthperformancewasstudiedTheresultsindicatedthatthecrudeextractofFicuscaricaLleaveswasinnocuousLDsowas149439／kg50g／kgddosagecangentlypromotethegrowt

8、hofmalemice，buthavenoeffectonfemalemiceAlldosagesofthecrudeextractofFicuscaricaLleaveshavenoeffectsondailyfeedintakesofrats50g&gddosageCanobviouslyincreasetheweightofmice’Sliver10eCkgddosagecanobviouslyincreasetheweighto

9、fmalemicethymus，butnoeffectonkidneyandspleenThecrudeextractofUsnea10ngissimawaslownoxious，LDsowas17669／kg10me#gdand50mgn(gddosagesCanobviouslyincreasetheweightofliulemalemice，andalsoCanpromotethegrowthoflittlefemalemice2

10、00mg／kgddosageCanrestrainthegrowthoflittlemaleandfemalemiceAlldosagesofthecrudeextractofUsnea10ngissimahavenoeffectsondaffyfeedintakesofrats10mg／kgd、50mg／kgdand200mg／kgdosagescanobservablyincreasetheweightofratliverespec