黑色素瘤抗原基因在胃癌組織中表達(dá)的研究.pdf

1、青島大學(xué)碩士學(xué)位論文黑色素瘤抗原基因在胃癌組織中表達(dá)的研究姓名：關(guān)向宏申請學(xué)位級別：碩士專業(yè)：病原生物學(xué)指導(dǎo)教師：羅兵2002.4.10英文摘要STUDY0NEXPRESSIONOFMELANoMAANTIGENGENESINGASTIUCCARCINoMABSTRACTobjectiveAsahumantumor—specificantigenthatcanberecognizedbyCTL，MAGEgenefamilyhashigh

2、expressionrateinvarioustumortissuesanditsproductofgeneexpressionhasgoodtumorspecificitywhichmakesitahotspotofresearchatpresentWestudiedtheexpressionofMAGE—l，一2and3genesin30samplesofhumangastriccarcinomasandtheassociation

3、betweentheexpressionsanddifferentiation，aswellastheexpressionsandclinicalstage，tryingtolaythetheoreticalandexperimentalfoundationforusingthesegenesproductsasthevaccinetoperformimmunotherapyonpatientsofgastriccarcinomaMet

4、hodsTheexpressionofMAGE12and一3genesin30gastriccarcinomasspecimensand30correspondingadjacentnormalgastrictissuespecimenswasdetectedbyareversetranscriptase———polymerasechainreaction(RTPCR)TheresultswereanalyzedaccordingtOc

5、linicopathologicfactorsofthetumorThe6samplesofRTPCRproductsweresequencedResultsMAGEgenesfamilyhashighexpressionrateingastriccarcinoma20(667％1ofthe30gastriccarcinomasspecimensexpressedatleastoneoftheMAGEmRNA，ofwhich14spec

6、imensexpressedMAGElmRNA，9specimensexpressedMAGE2mRNAand11specimensexpressedMAGE3mRNA，withthepositiveratesbeing467％，30O％，and367％respectivelyTherewasnosignificantstatisticdifferencebetweentheexpressionofthesethreeMAGEgenes

7、rx!=1796，P=0407005)TherewasnoexpressionofthesethreeMAGEgenesintheadjacenttissueofgastriccarcinomaThereWasmarkedsignificantstatisticdiffefencebetweentheexpressionratesofthethreeMAGEgenesintissueofcarcinomaandthoseintheadi