東方梨生理活性成分的HPLC分析研究.pdf

1、河北農業(yè)大學碩士學位論文東方梨生理活性成分的HPLC分析研究姓名：馬良申請學位級別：碩士專業(yè)：農產品加工及貯藏工程指導教師：崔同陳冠華20040612AnalysisoftheMajorActivityConstituentsinEasternPearbyHighPerformanceLiquidChromatographyMaLiangSpecialty：Agricultureproductprocessingandstorageen

2、gineeringAdviser：CuiTongAbstractChinaisnotonlythecradlecenterofeasternpearbutalsothebiggestcountyinpear’SoutputoftheworldDuringthelengthyprocessofgrowthandeating，sometherapyfunctionthroughbitingandsuppingandmanyhygienica

3、llytheancientsfoundedfunctionButtheinfrequentinvestigationsandreportsabouttheconstituentswithphysiologicactivityinpearfruitwerefoundedbyfarOurresearchteamfoundedtheupperlevelcontentoftheconstituentswithphysiologicactivit

4、yforexamplearbutinandchlorogenicacidineasternpearininitialstagesItwasfoundedthatArbutinandchlorogenicacidshouldbetherepresentativecomponentswithphysiologicactivityineasternpearinfactbyourtestIsocraticelutionreversedphase

5、highperformanceliquidchromatographic(HPLC)methodandgradientelutionreversedphasehigh—performanceliquidchromatographic(HPLC)methodfortheanalysisofthemainconstituentswithphysiologicactivityinpearfruitweredevelopedwithhigh—p

6、erformanceliquidchromatographicinstrumentwhichwasmadeinchinaIsocraticelutionmethodmakeupofareversedphasecolumnf46mmx200mm)filledwith5BmHYPERSILBDSasthestationaryphaseThemobilephasewasmadeofCH30H，H20(25：75，v／v)dualisticsy

7、stematpH320，whichadjusted，byphosphoricacidTheflowrateofmobilephasewas06ml／minDuringl3minutestheanalysiswasendedat280nmwavelengthwithultravioletdetectorinroomtemperatureThelinearitycorrelativecoefficientare099998botharbut

8、inandchlorogenicacidnlelinearrangeisat144。5000ngtoarbutinandisat200—5000ngtochlorogenicacidThetwocomponents’lowestdetectedlimitwasO153ngand0150ngrespectivelyGradientelutionreversed—phaseHPLCmethodusingtwopumpsestablished

9、tothemainpolyphenoliccompoundsforexamplearbutin，chlorogenicacid，catechin，epieatechin，caffeicacid，pcoumaricacid，quercetin，et，alinpearfruitGradientelutionprogramfmirdB％)：O／2，3／2，4／25，11／50，16／80，26／80，33／2，45／2Themobilepha

10、seatpH320adjustedbyphosphoricacidanddetectedat280nmwavelengthInthisHPLCsystem，themainactivecomponentswereseparatedwithin30minutesandchromatographycolumnconditionwasrenewablystabilizedwithin45minutesandthenextsampleCanber

11、unDynamicchangesinthemainconstituentswithphysiologicactivityduringtheflorescenceandthefruitsdevelopmentstageof“Ya’PearweremonitoredbythedevelopedHPLCmethodsThecontentsofthetwoconstituentsindifferentorgansduringthefloresc