中國粳稻核心種質(zhì)耐低磷種質(zhì)篩選及水稻耐低磷脅迫QTL定位.pdf

1、中國農(nóng)業(yè)大學(xué)碩士學(xué)位論文中國粳稻核心種質(zhì)耐低磷種質(zhì)篩選及水稻耐低磷脅迫QTL定位姓名：黃超申請學(xué)位級別：碩士專業(yè)：作物遺傳育種指導(dǎo)教師：李自超20050501ABSTRACTModemagricultureisnowdevelopingwithlessCOUSUmptionandlesspollutionandmoreeffectiveThistrendrequiresthebreedertodevelopvarietesthatcar

2、ltransformandintakethedepositoftheunsolvedphosphorusinthesoilbyitsownmetabolismIntheseyearsthatselectingandbreedingsomecropswhichcarltoleratelowphosphorusbecomesoneoftheadvancedresearchtopicsInthisresearch，somegetypeswer

3、escreenedfrom366typesofriceinthefieldAtthesametimewelocatetheQTLsfortolerancetolowphosphorususingl16DHlinesand120RILlineswhicharemadebyIRATl09andYuefuThemainresultsarethefollowing：1Accordingtothisexperiment’sresult，wedev

4、elopedthescreeningmethodfotmdedbyformerandusedtheretativevaluesteplacetheabsolutevalLieastheindexforscreeningthelinestolerancetothelowphosphorusWeselectedthefollowingtraitastheindexesfortolerancetothelowphosphorus：Seedpe

5、rpanicleandcalculatedthemeanoftherelativevaluesofthefivetraitsastheindexofscreeninglinestolencetolowphosphorusIntheendwedevidedallthelinesby5groups：tolencetolo、Ⅳphosphorus，nonsensitivetolowphosphorus，mediumtype，subsensit

6、ivetolowphosphorus，sensitivetolowphosphorus50lineswhichcantoleratelow—phosphoruswereintheresult：ChimaoNiandaozi，Zhongjin984—02，eta1Andthereare5llineswhichissensitivetoIowphosphorus：Xiangdao，Jingxil7，Qingzal，eta1Intheothe

7、rlinesthereare85nousensitivelines，92mediumlinesand86sub—sensitive1ines2AnalysingtheRILpopulationwhichwasculturedinuplandbyQTLMAPPER16software，wegottheresult：thereisamaingeneticlociwasidentified，whichislocateelinthechromo

8、some5withLOD378additivevalue_00407andcontribution00208Therefire2maingeneticlociswhicharecalculatedbythebiomassbeyondthelandandlocatedinchrosome10andchromosome12withadditivevalue00738and00948。LOD27land344、contribution0085

9、9and01418Thereare4pairsofepistasislociswhicharecalculatedBytillingnumberonepairofepistasislociiscalculated：RM297RMll83inchromosomelandRMl231一RMl244inchromosome1withtotaladdifivevalueO0175totalepistasisvalue00271，totalcon

10、tributionbyadditiveenviromnantinteractionO0107totalcontributionbyepistasis。environmentinteraction0056Thereare3pairsofepistasislociswhichareaffectedbyheight，whichrespectivelyareRMI13一RM493inchromosomelandRMl261一RMl246inch

11、romosome12，RM87RMl61inchromosome5andOSR29RM201inchromosome9RMl62一RM454inchromosome6andRM560oSR22inchromosome7Thetotalcontributionofadditivesis0Thetotalcontributionofepistasisis01365Thetotalcontributionofadditivexenvironm

12、entis00033Thetotalcontributionofepistasis“environmentis025513AccordingtotheDHpopulationwhichwereculturedinuplandfor2yearsapakofepistasislociswerecalculatedbytillingnumber，whichareC246G292inchromosome5andR617$826inchromos

13、ome12TheLODofthemis464andthetotalcontributionoftheadditivesis0andthetotalcontributionoftheepistasisisO086，Thecontributionofadditivexenvironmentinteractionis0madthecontributionofepistatic。environmentinteractionisO3372TheD