調(diào)控水稻時(shí)期轉(zhuǎn)變基因NECK LEAF1的定位、克隆及功能分析.pdf

1、中國科學(xué)院上海生命科學(xué)研究院植物生理生態(tài)研究所博士學(xué)位論文調(diào)控水稻時(shí)期轉(zhuǎn)變基因NECKLEAF1的定位、克隆及功能分析姓名：王麗萍申請學(xué)位級別：博士專業(yè)：遺傳學(xué)指導(dǎo)教師：羅達(dá)20060701英史摘要AbstractMappingc1011ingandfunctionaIanaIysisofNECKLEAFl(NL，)，aGATA一1iketranscriptionfactorregulatingphasetransitioninrice

2、deveIopmentLipingWangDirectedbyProfessorDaLuoPhasetransitioninfloweringplantsisageneticallyamcnabledevelopmentalprocess，whichinvolvesmodulationofthecomplexgeneticnetworkandalterationofthecharacteristicmorphologicalpatter

3、nsDuringthephasetransitionfromvegetativeteproductivestageinrice(Orysa，，ativa)，theidentityofshootapicalmeristem(SAM)isalteredandgivesrisetothedevelopmentofpaniclewithallleaf(bract)primordiaaborted，whilethedifferentialelon

4、gationofsevcratupperinternodesissynchronizedwiththepanicledevelopmentanddeterminesthefinalplantheightHere，wereportagroupofricemutantswhichwereallelictoageneticlocus，neckleafl(nil)，whichhasbeenservedasamorphologicalmarker

5、forricegeneticanalysisforlongtimeThesemutantsdisplayovergrownbractsonthepanicleandalteredelongationpatternoftheupperinternodesduringthereproductivephaseTheNLlgenewasisolatedthroughpositionalcloningapproach，whichencodesaG

6、ATAtypetranscriptionfactorwithasinglezincfingerdomainWedeterminedthesequenceofthecandidategencandidentifiedthenuc]eotidesequencewhichresultedinmutationinthreeallelesA62kbgenornicfragmentcontainingtheNLlcodingregion5’upst

7、reamsequenceand3’downstreamregionwasintroducedintothenll—Jmutant，confimfiagthattherill，mutantphenotypewasrescuedbythecaodidategene，InsituhybridizationexperimentrevealedthatNLlwasstronglyexpressedintheincipientprimordiaof

8、bracts，consistentwithitsmutanteffectswiththeoverdevelopcdbractsGeneticinteractionbetweenNLIandPLASTOCHRON，(PLA／)，aknowngeneplayinganimportantroletegulateleafinitiafion，wasconductedItwasfoundthatthephenotypeofniltr2doublc

9、mutantwasmimickingtothatoftr2mutant，andtheexpressionpatternof|DL。4』wasalteredinrillmutantwhileNLtexpressionwasnotaffectedintr2mutant，indicatingthatbothNLlshouldplayaroJeinthecontrolofpanicledevelopmentthroughPLA]although