四個AP2-EREBP類轉錄因子的功能分析.pdf

1、Y1132688女a——丑_ⅡR#目SY200^0032(⑧面欺農林釬捉大學2007屆攻讀碩士學住研咒生學位(畢業(yè))論文四個AP2／EREBP類轉錄因子的功能分析學科專業(yè)研究萬向研究生指導教師完成時間韭塑巡焦直型，壅些生塑堇盛————盤—蘊——匿耀些塑攫2蝕!笙旦中目映日揚凌THESTUDYONBIOLOGICALFUNCTIONOFFOURAP2／EREBPTRANSCRIPTIONFACTORSABSTRACTPlantcellha

2、saverycomplexsigmatransfermechanismInbioticandabioticstressestheyactivateadiversesetofphysiological，metabolicanddefensesystemstosurviveandtosustaingrowthTranscriptionfactorsplayveryimportantrolesinregulatedthegrowthdevel

3、opmentsignaltransductionandgeneexpressionAP2／EREBPisagroupoftranscriptionfactors，specialinplants玨eyareinducedbybioticandabioticstresses，andveryimportantinstressrelatedtranscdption$WeisolatedfourtranscriptionfactorsERFl，E

4、RF2andDREB3／DREB4whichfromwheat(Xiaobaimai)cDNAlibraryindroughtstressnle內urtranscriptionfactorsareallneverbereportedbeforeThestudyweworkedbeforeshowedthatERF，／ERF2WaSinducedbybioticandabioticstressesandDREB3／DREB4wereonl

5、yinducedbyabioticstressesInthisstudywewantedtOidentifythebiologicalfunctionsofthefourgenes，throughthetransgenicoverexperssionplantsProgressesofthisresearchalefollowing：1TheanalysisoftheERFI／ERF2transgenictobaccosinsaltst

6、ress：TheToERFI／ERF2transgenictobaccolinesweretreatedinsaltstressfor50daysTheresultshowedthattheERFl／ERF2improvedthesalttoleranceoftransgenictobaccos2TheanalysisoftheERFI／ERF2transgenictobaccosinpathogensstress：T1leT0ERFl

7、／ERF2transgenictobaccolineswereinoculmedPseudomonassyringae肼TabaciatsixtosevenleavesoldThestudyindicatedtheERFl／ERF2Increasedtolerancetoinfectionbypathogen3TheeffectoftheERF2tOtransgenictobaccosstomas：Thestomasoftransgeh

8、ictobaccosclosedquicklyinbothdroughtandABAtreatment41rI圮analysisoftheDREB3／DREB4transgenicwheatsindroughtstress：ThedroughttoleranceoftheDREB3fDREB4transgenicwheatsWere、dentifiedbycontro、theWaterDREB3／DREB4changedthephysi

9、ologicalandmetabolicparametersindroughtconditionsimprovedthetransgenicwheat’StoleranceInthisstudyWeanalyzedthefunctionofERFI／ERF2andDREB3／DREB4insaltandpathogenstress7n蛇resultsindicatedERFlfERF2WaSrelamdtobioticandabioti