甲狀腺良惡性結(jié)節(jié)的臨床病理學(xué)研究.pdf

1、石河子大學(xué)碩士學(xué)位論文甲狀腺良惡性結(jié)節(jié)的臨床病理學(xué)研究姓名：肖榮霞申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：病理學(xué)與病理生理學(xué)指導(dǎo)教師：趙瑾20090501AbstractO—bjec—tiv—e—：Inordertoprovidemarkerofdiagnosisanddifferentiateddiagnosisofbenignandmalignantthyroidnodules，wedetectedtheexpressionofHMGA2mRNAa

2、ndHMGA2protein、Galectin一3、CD44v6andMMP2proteininbenignandmaligantthyroidnodulesMothods：1l5casesofparaffin—embeddedtissueofthyroidnoduleswerecollectedfromarchivesofPathologyinShiheziUniversitySchoolofMedicineandthePeople’

3、SHospitalofUrumqiAutonomousRegionandTheHospitalofXinJiangmilitaryareafrom1983to2008，including39casesoffollicularcarcinoma(FTC)，17casesoffollicularvarintofpapillarycarcinoma(FVPTC)，18casesofpapillarycarcinoma(FTC)，22Cases

4、offollicularadenoma(FTAland19casesofadenomatOUSnodulargoiter30casesofsurgeryfreshspeciment1softhyroidnoduleswerecollectedfromApri2007toJanuary2009including14casesofpapillarycarcinoma5casesofFTAand1lcasesofnodulargoiterA1

5、1caseswerestainedbyHEstaininganddiagnosedbymorethantwoseniorpathologicalprofessorsreferringtothe2004WHOclassificationstandardswedetectedtheexpressionofHMGA，、Galectin3、CD44v6andMMP一2proteininbenignandmalignantthyroidnodul

6、esbyimmunohistochemicalstainingandtheexpressionofHMGA，genemRNAin45casesofbenignandmalignantthyroidnodulesbyreversetranscriptasepolymerasechainreaction(RTPCR)A11dateswereanalysisbysoftwareSPSS130andcombiningwithclinicalan

7、dpathologicaldatatoanalysisitResu1ts：ThepositiverateofHMGA2、CD44v6、MMP2andGalectin一3proteinexpressioninbenignandmalignantthyroidnodulewas1220％、1463％、1707％、1220％and4595％、7297％、7297％、6486％respectivelyTherewassignificantdif

8、ferenceofthefourmarkersexpressionbewteenbenignandmalignantgroupWhilethepositiverateofHMGA2、CD44v6、MMP一2andGalectin3proteinexpressioninFTCandFTAwas3077％、6154％、7179％、6154％and1364％、1364％、1819％、1364％，respectivelyTherewassign

9、ificantdifferencebetweenFTCandFTAgroupCD44v6、MMP2andGalectin一3proteinexpressionwassignificantlycorrelatedwithlymphnodemetastasisHMGA2mRNAexpressionof45casesofthyroidnodulesbyRrrPCRshowedthatHMGA2mRNAexpressionin9casesofF

10、TA11casesofnodulargoiter25casesofthyroidcarcinomawas013019、055035and105036respectivelyConclusion：(1)HMGA2、CD44v6、MMP2andGalectin3proteinareusefulmarkersindiagnosisanddifferentialdiagnosisbetweenbenignandmalignantthyroidn

11、odulesbasedonthemorphologicalcharacteristicsWhiletheirexpressionishelpforthediagnosisanddifferentiateddiagnosisonFTCandFTA(2)TheexpressionofCD44v6、MMP一2、Galectin一3proteinisassociatedwithinvasionandmetastasisofthyroidcarc