2023年全國碩士研究生考試考研英語一試題真題(含答案詳解+作文范文)_第1頁
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1、河北農(nóng)業(yè)大學(xué)碩士學(xué)位論文四倍體葡萄誘導(dǎo)技術(shù)的研究姓名:馬愛紅申請學(xué)位級別:碩士專業(yè):果樹學(xué)指導(dǎo)教師:孫建設(shè)李紹華20050621StudyonInduclionofTetrapioidGrapesAuthor:MaAi—hungMajor:PomologyTuter:SunJiansheandLiShao—hua(DepartmentofHorticulture,AgriculturalUniversityofHebei,Banding

2、,China071001)AbstractlnductionsoftetraploidgrapeswereconductedwithcolchicinefrominvitroVitisviniferacv‘Jingxiu’‘RedGlobe’,‘Jingzaojing’,‘Rizamat’,‘ManicureFinger’,‘RedCocoon’,and‘YaIatiRosa’Ploidylevelsweredeterminedbyfl

3、owcytometryToexploretheeffectivemethodsandoptimaltreatmentsofinductiontetraploids,comparisonsofsoakingshootsegmentsincolchicinesolutionandaddingcolchinineintheculturemediumweredonewith‘Jingxiu’aloneTheeffectsofbothdurati

4、onofsoakingandconcentrationofcolchicineonefficiencyofinductionwerestudiedThelltetraploidgrapeswerefurtherinducedfrom‘3ingxiu’and‘RedGlobe’withanoptimaltreatmentFinally,fivecuhivars,including‘Jingzaojing’,‘Rizamat’,‘Manic

5、ureFinger’,‘RedCocoon’,and‘YatomiRosa’,wereinducedtetraploidsMainresultsareasfoIlows:1Thesoaked—treatmenthadincreasedefficiencyofinducingtetraploidsthanoneofaddinginmediumItwascharacterizedassimpleoperation,lesscolchicin

6、eusageandeffectiveinductionofbothpuretetraploidsandchimeradueiocellularinfiltrationeasilybysoakingOnthecontrarythetreatmentofaddiagcolchicinesintheculturemediumwithdifferentagesofinvitro‘Jingxiu’waspooreffectofinductiono

7、ftetraptoids,2TheeffectsofcolchicinenoinhibitingplantgrowthdifieredinconcentrationofcolchicineanddurationofsoakingstemfragmentsincolchicinesolutionTheinhibitionofplantletsgrowthwasenhancedwithsoakedtimeextensionandthecon

8、centrationincreaseDeathofplantletswasobservedwhentheconcentrationofcolchicineswashigherthan2000mgL‘orthedurationofsoakingwaslongerthan48hoursTileincidenceofdeathofplantletsmostlywashigherthan30%whentheconcentrationwashig

9、herthan40()0mgL1Thedeathrateof‘Jingxiu’and’RedGlobe’wasmorethan70%astreatedat4000mgL1for4d3Soakingshootsegmentswithcolchicineat2000mg‘L1for3to4dor3000mgL14000mg‘L~for2to4dinducedpuretetraploidsduringfirstregenerationAdes

10、iredrangeof167%to23。3%oftetraploidswasobtainedbysoakingatcolchicinesconcentrationof3000mgL~for3Io4daysHowevercnlchicineconcentrationsof2000mg‘L1orlowerandsoakingfor48horshorterinducedpuretetraploidsaftertwotofiveregenera

11、tionseventhoughtetraploidswerenotobservedduringfirstregeneration4Ploidyofplantletwasnotchangedthroughgrowing。butseparationofchimeraoccuffedduringregenerationSomemorphologicalfeaturesofleavesandrootswereobviouslychangedwh

12、ilechimerasbecameintopuretetraploidsThesechangeswerestronglyrelatedtoeithertheconcentrationofcolchicineordurationofsoakediothecolchicinesolution,511heefficiencyofinduetionvariedwithdifferenteultivatsTheoptimaltreatmentco

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