雌激素、大豆異黃酮對(duì)去卵巢大鼠骨代謝影響的研究.pdf

1、山西醫(yī)科大學(xué)碩士學(xué)位論文雌激素、大豆異黃酮對(duì)去卵巢大鼠骨代謝影響的研究姓名：藥翼華申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：婦產(chǎn)科學(xué)指導(dǎo)教師：郭述真20080404山西醫(yī)科大學(xué)碩士學(xué)位論文ABSTRACT【Objective】Tostudytheeffectofsoybeanisoflavonesonthebonemetabolismovariectomizedrats[Methods]Twentyeight10weekoldfemaleSpragueD

2、awley(SD)ratswererandomlyassignedtothefollowingfourexperimentalgroups：1Shamoperategroup(SHAM)2Ovariectomizedgroup(ovx)3Ovariectomizedaddedsoybeanisoflavonesgroup(OVXISO)Ovariectomizedandfedorallysoybeanisoflavonesusingas

3、tomachtube(100mg／kg／d)4Ovariectomizedaddedestradiol(OVXE)Ovariectomizedandintramuscularlyinjectedwithestradiolbenzoate(O1mg／kgonceaweek)RatsoftheSHAMgrouponlyunderwenttheresectionofabitfatTherestgroupsallunderwenttherese

4、ctionofbilateralovariesandfedphysiologicalsalinc(NS)onaequalleverwithsoybcanisoflavonesThetimeofexperimentis12weekThenalltheratswerekilledbybloodlettingoffemoralarterytoexaminetheactivitiesofserumtotalalkalinephosphatase

5、(ALP)，bonealkalinephosphatase(BALP)andtartrateresistantacidphosphatase(TRACP)Toexamineurinecalcium，phosphoruscompared、)I，inlcreatinineandpyridinolinefPyd)leverinratsSeparatingtherightfemurofalltheratstoexaminetheminerald

6、ensity(BMD)andthecalciumcontentoftheboneResults：RatsintheOVXgrouphadsignificantlylowercalciumandphosphoruscontentandmineraldensityofthebone(BMD)(Po05)，andhigherserumcalciumlever(Po05)，andhigherurinecalciumandphosphorusco

7、mparedwithcreatinineandpyridinoline(Pyd)leverinratsthanthoseinothergroups口o05)Andserumcalciumandurinaryexcretionofcalciumandpyridinoline(Pyd)leverhadsignificantlylowerinOVXISO，OVXEgroups(Po05)，andhigherthemineraldensity(

8、BMD)andthecalciumcontentoftheboneinOV》cISO，OVXEgroups(P005)TheactivitiesofALPBALP，TRACPandtheIL6contentoftheseruminOVXgrouphadsignificantlyhigher(PO05)AndallhadlowerinOVXISO，OVXEgroups口005)[Conclusion]SoybeanisoflavonesC

9、aninhibitthebonelossofovariectomizedrats，andenhancecalciumabsorption，anddecreasebonemetabolismTheseresultsindicatethatsoybeanisoflavonesexhibitsestrogenicaction[Keywords]Soybeanisoflavones，F(xiàn)emalerats，Ovaryresection，Bonem