擬南芥ckrc突變體的篩選和初步研究.pdf

1、蘭州大學(xué)碩士學(xué)位論文擬南芥ckrc突變體的篩選和初步研究姓名：武磊申請學(xué)位級別：碩士專業(yè)：生物學(xué)細胞生物學(xué)指導(dǎo)教師：郭光沁20100501蘭州大學(xué)碩士學(xué)位論文擬南芥ckrc突變體的篩選和初步研究AbstractThisworkisbasedonthemutantckrcl1(￡yto_lanininducedroot_curlin91一l、)isolatedpreviouslybyourresearchgroupCKRClencodes

2、_tryptophan_aminetransferaseinA__rabidopsis(TAA)，catalyzingtheformationofIPAfromtryptophanthroughitsoxidativeaminetransferringactivitythefirststepoftheIPApathwayforL認biosynthesisckrclJhastypicallowauxinphenotypes，anddisp

3、layscurlingrootswhenplacedonthecytokinincontainingmediumConsideringmultiplepathwaysof姒synthesisandtheircomplexmolecularregulationitshouldbefeasibletogetmorenovelnonallelicmutantshavingthesamephenotypeaSckrcl，Moleculargen

4、eticandbiochemicalstudiesonthesemutantswouldprovidenewinsightintothecomplicatedbiologyofauxininplantgrowthanddevelopmentByscreening120，000seedlingsinabout40，000TDNAinsertedlinesweisolated5rootcurlingmutants，whichCanbecla

5、ssifiedinto2groups：rootcurling(rclcurlingwithoutexogenouscytokinin)andcyto_舫nininducedroot_curling(ckrc)Theformerincludes023(rcll—l、、024(rcl21and069(rcl31andthelatterincludes052(ckrc2I、)和066(ckrc3J)SequenceanalysisoftheT

6、ailPCRproductsshowedthattheTDNAinsertedsitesofmutant023(rcllJ)052(ckrc2J)and069(rcl3一J『)arethe3UTR(_untranslatedregions)ofAT2939990【encodeseukaryotictranslationinitiationfactor2(EIF2)]，the6thintronofAT2938120(AUXI)andthe

7、10也intronofATl901650(encodespeptidase)，respectivelyChemicalcomplementationwithdifferentauxinsandIPAtothe5mutantsshowedthatthephenotypesofsomemutantsCanberescuedtovariousextentsbythesechemicalsGeneticanalysisindicatedthat