13462.生物條形碼信號(hào)放大技術(shù)檢測(cè)食源性致病菌dna的研究

1、原創(chuàng)性聲明本人聲明所呈交的學(xué)位論文是我個(gè)人在導(dǎo)師指導(dǎo)下進(jìn)行的研究工作及取得的研究成果。盡我所知，除了文中特別加以標(biāo)注和致謝的地方外，論文中不包含其他人已經(jīng)發(fā)表或撰寫(xiě)過(guò)的研究成果，也不包含為獲得寧夏大學(xué)或其他教育機(jī)構(gòu)的學(xué)位或證書(shū)而是用過(guò)的材料。與我一同工作的同志對(duì)本研究所做的任何貢獻(xiàn)均已在論文中作了明確的說(shuō)明并表示了謝意。研究生簽名：時(shí)間：知，多年支月糾同關(guān)于論文使用授權(quán)的說(shuō)明本人完全了解寧夏大學(xué)有關(guān)保留、使用學(xué)位論文的規(guī)定，即：學(xué)校有權(quán)

2、保留送交論文的復(fù)印件和磁盤(pán)，允許論文被查閱和借閱，可以采用影印、縮印或掃描等復(fù)制手段保存、匯編學(xué)位論文。同意寧夏大學(xué)可以用不同方式在不同媒體上發(fā)表、傳播學(xué)位論文的全部或部分內(nèi)容。(保密的學(xué)位論文在解密后應(yīng)遵守此協(xié)議)研究生簽名：與—9d冶恕時(shí)間：抽，弓年支月乙，同翮簽名：粕擔(dān)兩時(shí)問(wèn)：c乙，弓年主月LIFtbiobarcodeEscherichiaAbstractthesis，Weconstructanovel，rapidandsensi

3、tivedetectionmethodforDNAbasedontechnologybyelectrochemicalanalysis，andrealizedaccurateandeffectivedetectionofcoliDNA，SalmonellaDNA，ShigellaDNAatthesametimeThemaincontentsareasfoIlOWS：(1)Thedifferenceinelectrochemicalbeh

4、aviorbetweenGrichDNAandordinaryDNAwereresearchedinthefirstworkAcomparativestudyonthefourtypesofDNAintheelectrochemicalbehaviorsuchasordinarysinglestranded(ss—DNA)，doublestrandDNA(dsDNA)，singleanddoublestrandG—richDNAAgol

5、dnanoparticlesarrayelectrodewasmadebyelectro—depositiontechnique，whichwasfoundnotonlycouldefficientlyimprovedtheelectroderesponsesignal，butalsocouldkeepingthestabilityoftheelectrodesTheresultindicatethatthismethodcaneasy

6、andefficientlyrealizethedetectionoftargetDNAsample，aswellasdistinguisholigonucleotidesofwhichhavedifferenceintheirbasetype(2)TheEscherichiacoligenesequencesandthetargetDNAmoleculesweredeterminedbasedontheliteraturesandth

7、erelateddatabase，anddisignedtheDNAprobesTheanalogcomputationiscarriedoutbasedonprofessionalsoftware，forcheckingthefeasibilityofthedesigedDNAprobesThedetectionofEscherichiacoliDNAwassimplyrealizedwiththeimprovedtwostepmet

8、hodbytheamplificationtechniqueusingbio—barcodesandgoldnanoparticles(3)DeterminingthecharacteristicgenesequencesandthetargetDNAmoleculesofSalmonellaandShigellabasedontheliteraturesandtherelateddatabase，andthendisignedtheD

9、NAprobesTheanalogcomputationiscarriedoutbasedonprofessionalsoftware，forcheckingthefeasibilityofthedesigedDNAprobesAnoveldetectionmethodwasconstructedbylabelfreedirectelectrochemicalanalysisformultipletargetscontainingthe